Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:5000Application
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody diluted at 1:100000.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin antibody at 1:500 dilution.Application
alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody diluted at 1:5000.| Product Name | Alpha 1 Microglobulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Alpha 1 Microglobulin (protein AMBP)This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha-1-Microglobulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | CatCat (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | A1M antibody AMBP antibody EDC1 antibody HCP antibody HI30 antibody IATIL antibody ITI antibody ITIL antibody ITILC antibody UTI antibody alpha-1-microglobulin antibody bikunin precursor antibody alpha-1-microglobulin/bikunin precur |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:5000
Application
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody diluted at 1:100000.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin antibody at 1:500 dilution.
Application
alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody diluted at 1:5000.| Positive Control | 293T , A431 , human plasma , HepG2 conditioned medium |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
