Application
Anti-α 1,2-Mannosidase IA : Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat pancreas at 0.2 μg/mL.Application
HepG2 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA . The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.Application
Whole extract of HepG2 cells was separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-α1,2-Mannosidase IA .Application
Whole extract of NIH3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-α1,2-Mannosidase IA . Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + immunizing peptide ( immunizing peptide : amino acids 138-154 of Human alpha 1,2 Mannosidase IA)Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse kidney at 0.4 μg/mL.Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse testis at 0.4 μg/mL.Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.| Product Name | alpha 1,2 Mannosidase IA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha 1,2 Mannosidase IAAlpha 1,2-Mannosidase IA is a Type II transmembrane Golgi-resident enzyme that belongs to class I alpha 1,2- Mannosidases (glycosylhydrolase family 47). Alpha 1,2-Mannosidases play an essential role in the maturation of N-glycans to hybrid and complex oligosaccharides in mammalian cells. They can be classified into three subgroups according to their enzymatic activities. The second subgroup includes mammalian Golgi alpha 1,2-Mannosidases IA, IB, and IC. It has been suggested that Golgi mannosidases from this subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins. |
| Immunogen | Synthetic peptide: TLQKLPEEIQRDILLEK with N-terminal added cysteine, conjugated to KLH, corresponding to amino acids 138-154 of Human alpha 1,2 Mannosidase IA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAN9 HUMM3 alpha 1 2 Mannosidase IA alpha1 2 Mannosidase IA HUMM9 |
|---|---|
| Target | alpha 1,2 Mannosidase IA |
| Cellular Localization | Golgi Apparatus; Golgi apparatus membrane; single-pass type II membrane protein |
Application
Anti-α 1,2-Mannosidase IA : Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat pancreas at 0.2 μg/mL.
Application
HepG2 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA . The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Application
Whole extract of HepG2 cells was separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-α1,2-Mannosidase IA .
Application
Whole extract of NIH3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-α1,2-Mannosidase IA . Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + immunizing peptide ( immunizing peptide : amino acids 138-154 of Human alpha 1,2 Mannosidase IA)
Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse kidney at 0.4 μg/mL.
Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse testis at 0.4 μg/mL.
Application
Anti-α 1,2-Mannosidase IA: Cat. No. Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.| Positive Control | Whole extract of Mouse NIH 3T3 cells; Human HepG2 cells; Rat NRK cells |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01% PBS, pH 7.4 |
