Application
alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term dilution: 1:500.Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 antibody at 1:500 dilution.| Product Name | alpha Actinin 4 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (85%), Xenopus laevis (92%), Chicken (94%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACTININ-4 antibody DKFZp686K23158 antibody FSGS antibody FSGS1 antibody ACTN4 antibody focal segmental glomerulosclerosis 1 antibody F-actin cross-linking protein antibody non-muscle alpha-actinin 4 antibody alpha-actinin-4 antibody actinin alpha4 |
|---|---|
| Molecular Weight(MW) | 105 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term dilution: 1:500.
Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , PC-12 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
