| Product Name | alpha Actinin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [0.T.02] to alpha ActininAlpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini. |
| Immunogen | Human alpha Actinin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 0.T.02 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC: Use at a concentration of 15 μg/ml. IHC: Use at a concentration of 15 μg/ml. This concentration gives positive immunostaining for alpha Actinin in MRC5 cells fixed with methanol. A previous lot of antibody detected alpha Actinin in frozen sections. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 103 kDa. Detects alpha Actinin in RIPA lysates from human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-alpha Actinin (1mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Is unsuitable for IP. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 102575 87 ACTN1 P12814 alpha Actinin |
|---|---|
| Target | alpha Actinin |
| Tissue Specificity | Recognizes and is specific for human alpha Actinin at 100kD |
| Cellular Localization | Cytoplasmic |
| Positive Control | RIPA lysates from human A431 cells (GTX82919) |
|---|---|
| Application Notes | ICC: Use at a concentration of 15 μg/ml. IHC: Use at a concentration of 15 μg/ml. This concentration gives positive immunostaining for alpha Actinin in MRC5 cells fixed with methanol. A previous lot of antibody detected alpha Actinin in frozen sections. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 103 kDa. Detects alpha Actinin in RIPA lysates from human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-alpha Actinin (1mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Is unsuitable for IP. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% Sodium Azide |
