Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded SG xenograft , using Alpha-adducin antibody at 1:100 dilution.Application
alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE alpha Adducin antibody [N1N2], N-term dilution: 1:1000| Product Name | alpha Adducin antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Adducin (adducin 1 (alpha))Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (98%), Zebrafish (89%), Xenopus laevis (93%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADDA antibody MGC3339 antibody MGC44427 antibody ADD1 antibody alpha-adducin antibody erythrocyte adducin alpha subunit antibody adducin 1 (alpha) antibody |
|---|---|
| Molecular Weight(MW) | 81 kDa (note) (6) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell membrane |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SG xenograft , using Alpha-adducin antibody at 1:100 dilution.
Application
alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE alpha Adducin antibody [N1N2], N-term dilution: 1:1000| Positive Control | Jurkat , Raji , NCI-H929 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
