Application
Western blot analysis of Alpha-crystallin B (pS19) expression in MCF7 (A); mouse spleen (B); mouse eye (C); rat liver (D); rat eye (E) whole cell lysates.| Product Name | alpha B Crystallin (phospho Ser19) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha B Crystallin (phospho Ser19)Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-crystallin B. The exact sequence is proprietary. |
| Modification | p-Ser19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSPB5 CTPP2 CMD1II crystallin alpha B HEL-S-101 MFM2 CRYAB CTRCT16 CRYA2 |
|---|---|
| Target | alpha B Crystallin (phospho Ser19) |
| Tissue Specificity | Recognizes endogenous levels of Alpha-crystallin B (pS19) protein. |
Application
Western blot analysis of Alpha-crystallin B (pS19) expression in MCF7 (A); mouse spleen (B); mouse eye (C); rat liver (D); rat eye (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
