Rabbit Polyclonal antibody to alpha cateninAssociates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.
Immunogen
Peptide sequence around aa. 639~643 (D-D-S-D-F) derived from Human alpha catenin.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Western blot analysis of extracts from Hela and 293 cells using a-catenin (Ab-641) antibody
Application
Western blot analysis of extracts from Hela and 293 cells using alpha -catenin antibody .
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.