| Product Name | alpha Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha CateninThe catenins (alpha, beta and gamma) are ubiquitously expressed, cytoplasmic proteins that associate with E cadherin. Alpha catenin can also bind P cadherin and N cadherin. Alpha Catenin exhibits both actin binding and bundling activities. Cadherin / catenin complexes are linked to the cytoskeleton via a direct association between alpha actinin and alpha Catenin. Increased tyrosine phosphorylation can disrupt catenin cadherin complexes, influencing cellular adhesion. |
| Immunogen | A synthetic peptide from the C-terminus of human alpha catenin protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| For IP: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For WB: Use at a concentration of 5-10μg/ml for 2hrs at RT. This antibody detects a band of ~ 102 kDa, which corresponds to the predicted molecular weight of alpha Catenin. IHC-P: Use at a concentration of 2.5-5μg/ml for 2hrs at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 mins. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseXenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | alpha Catenin 1495 FLJ36832 CTNNA1 CAP102 116805 P35221 alpha catenin antibody alpha catenin |
|---|---|
| Target | alpha Catenin |
| Cellular Localization | Cytoplasmic and cell membrane. |
| Positive Control | MCF-7 and A437 cells. Breast carcinoma tissue sections |
|---|---|
| Application Notes | For IP: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For WB: Use at a concentration of 5-10μg/ml for 2hrs at RT. This antibody detects a band of ~ 102 kDa, which corresponds to the predicted molecular weight of alpha Catenin. IHC-P: Use at a concentration of 2.5-5μg/ml for 2hrs at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 mins. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM Phosphate-buffered saline, pH 7.2, containing 0.2%BSA, 0.09% sodium azide |
