Application
WB analysis of extracts from various cell lines using AFP antibody.Application
ICC/IF analysis of HeLa cells using AFP antibody (green) and DAPI (blue).Application
IHC-P analysis of human stomach cancer using AFP antibody at a dilution of 1:200.Application
IHC-P analysis of human bladder cancer using AFP antibody at a dilution of 1:200.| Product Name | Alpha fetoprotein / AFP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AFPThis gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| Immunogen | C term -peptide of human AFP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:10 - 1:100.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FETA alpha-fetoprotein HPAFP |
|---|---|
| Target | AFP |
| Molecular Weight(MW) | 65 kDa (note) |
Application
WB analysis of extracts from various cell lines using AFP antibody.
Application
ICC/IF analysis of HeLa cells using AFP antibody (green) and DAPI (blue).
Application
IHC-P analysis of human stomach cancer using AFP antibody at a dilution of 1:200.
Application
IHC-P analysis of human bladder cancer using AFP antibody at a dilution of 1:200.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:10 - 1:100.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
