Application
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [ - Mixed cultures of rat CNS cells stained with (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.| Product Name | alpha Internexin antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to alpha-Internexin [1D2]alpha-Internexin is a ~66 kDa Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. |
| Immunogen | GTX30664 was raised against purified recombinant rat alpha-internexin expressed in and purified from E. coli. |
| Clonality | Monoclonal |
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| Clone Number | 1D2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| For WB - Use at 1:10,000 (ECL) : |
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| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Gene Synonyms | NF-66 NF66 NEF5 alpha Internexin MGC12702 9118 TXBP-1 Q5SYD2 INA 605338 TXBP1 NF 66 TXBP 1 |
|---|---|
| Target | alpha Internexin |
| Tissue Specificity | specifically recognizes the C-terminal non-helical extension of a-internexin. |
Application
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [ - Mixed cultures of rat CNS cells stained with (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.| Application Notes | For WB - Use at 1:10,000 (ECL) : |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.5 ml of tissue culture supernatant. Contains 5mM sodium azide. |
