| Product Name | alpha Smooth Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [0.N.5] to alpha Smooth Muscle ActinThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Immunogen | Synthetic peptide: MCEEEDSTAL, corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 0.N.5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-FIHC-P |
| IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 0.5-1 µg/ml. IHC-Fr: Use at a concentration of 0.5 µg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBaboonRabbitBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ACTA2 ACTSA MYMY5 AAT6 alpha SMA antibody Smactin antibody alpha SM actin antibody ASMA antibody aortic smooth muscle Alpha-actin-2 antibody actin aortic smooth muscle antibody cell growth inhibiting gene 46 protein antibo |
|---|---|
| Target | alpha Smooth Muscle Actin |
| Tissue Specificity | Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Smooth muscle or leiomyosarcoma. |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 0.5-1 µg/ml. IHC-Fr: Use at a concentration of 0.5 µg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 0.2% BSA, PBS. pH 7.4 |
