Application
Formalin fixed paraffin embedded human leiomyoma stained with Smooth Muscle Actin antibody (cat. # ).| Product Name | alpha Smooth Muscle Actin antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1A4] to alpha Smooth Muscle ActinThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Immunogen | Alpha-smooth muscle actin N-terminal decapeptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1A4 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.: |
|
| Species Reactivity | PigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACTA2 ACTSA MYMY5 AAT6 alpha SMA antibody Smactin antibody alpha SM actin antibody ASMA antibody aortic smooth muscle Alpha-actin-2 antibody actin aortic smooth muscle antibody cell growth inhibiting gene 46 protein antibo |
|---|---|
| Target | alpha Smooth Muscle Actin |
| Tissue Specificity | This antibody is specific to alpha-smooth isoform of actin. It reacts with smooth muscle cells of vessels and different parenchymes. This antibody does not cross-react with beta and gamma-cytoplasmic, alpha-sarcomeric and alpha-myocardial actin isoforms. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | Kappa |
Application
Formalin fixed paraffin embedded human leiomyoma stained with Smooth Muscle Actin antibody (cat. # ).| Positive Control | Leiomyoma |
|---|---|
| Application Notes | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate buffered saline containing 1%BSA and 0.09% sodium azide |
