Application
ELISA analysis of control antigen and antigen using alpha Smooth Muscle Actin antibody [4A4].Application
WB analysis of (1) HeLa, (2) A431, (3) Jurkat, (4) K562, (5) HEK293, (6) HepG2, (7) NIH/3T3, (8) PC-12 and (9) Cos7 lysates using alpha Smooth Muscle Actin antibody [4A4].Application
IHC-P analysis of human duodenum tissues (left) and human esophagus tissues (right) using alpha Smooth Muscle Actin antibody [4A4].Application
ICC/IF analysis of HepG2 cells using alpha Smooth Muscle Actin antibody [4A4] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of HeLa cells using alpha Smooth Muscle Actin antibody [4A4] (green) or a negative control (purple).| Product Name | alpha Smooth Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4A4] to alpha Smooth Muscle ActinThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Immunogen | The immunogen corresponding to human ACTA2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4A4 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTA2 ACTSA MYMY5 AAT6 alpha SMA antibody Smactin antibody alpha SM actin antibody ASMA antibody aortic smooth muscle Alpha-actin-2 antibody actin aortic smooth muscle antibody cell growth inhibiting gene 46 protein antibo |
|---|---|
| Target | alpha Smooth Muscle Actin |
| Molecular Weight(MW) | 42 kDa (note) |
Application
ELISA analysis of control antigen and antigen using alpha Smooth Muscle Actin antibody [4A4].
Application
WB analysis of (1) HeLa, (2) A431, (3) Jurkat, (4) K562, (5) HEK293, (6) HepG2, (7) NIH/3T3, (8) PC-12 and (9) Cos7 lysates using alpha Smooth Muscle Actin antibody [4A4].
Application
IHC-P analysis of human duodenum tissues (left) and human esophagus tissues (right) using alpha Smooth Muscle Actin antibody [4A4].
Application
ICC/IF analysis of HepG2 cells using alpha Smooth Muscle Actin antibody [4A4] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of HeLa cells using alpha Smooth Muscle Actin antibody [4A4] (green) or a negative control (purple).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
