| Product Name | alpha SNAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4E4] to alpha SNAPSNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNAP. a-SNAP (or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. alpha SNAP is expressed in all mammalian tissues. |
| Immunogen | Recombinant full length Human alpha SNAP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E4 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at an assay dependent dilution. IP: Use at a concentration of 2 - 10 μg/ml. WB: Use at a concentration of 0.5 - 5 μg/ml. Detects a band of approximately 36 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | 603215 P54920 alpha SNAP 8775 SNAPA NAPA |
|---|---|
| Target | alpha SNAP |
| Tissue Specificity | Does not cross react with beta-SNAP. |
| Cellular Localization | Cytoplasmic peripheral membrane protein |
| Positive Control | Rat kidney, brain and MDBK cells |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at a concentration of 2 - 10 μg/ml. WB: Use at a concentration of 0.5 - 5 μg/ml. Detects a band of approximately 36 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5 |
