| Product Name | alpha Syntrophin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha SyntrophinThe syntrophins are a family of intracellular peripheral membrane proteins (58-60 kDa), comprising at least three isoforms alpha1, beta1 and beta2. Syntrophins in mammalian skeletal muscle have been shown to be part of a complex of proteins associated with dystrophin, the product of Duchenne/Becker muscular dystrophy gene. The dystrophin-associated protein (DAP) complex is thought to link cortical actin to the extracellular matrix, thereby stabilizing the sarcolemma during repeated cycles of contraction and relaxation.6 At the neuromuscular junction, the DAPs have been implicated in agrin-stimulated clustering of nicotinic acetylcholine receptors. Dystrophin and DAPs are also found at synapses in the brain and retina. Thus, the syntrophins and other DAPs may participate in sarcolemma stabilization and synaptogenesis. Based on their domain organization and association with neuronal nitric oxide synthase (nNOS), syntrophins are thought to function as modular adapters to recruit signaling proteins to the membrane via association with the dystrophin complex. The alpha1, beta1 and beta2-syntrophin isoforms contain two pleckstrin (PH) homology domains, found in a wide array of signaling proteins, mediating signal-dependent membrane association. Inserted within the first syntrophin PH domain is a PDZ domain, which is known to be involved in binding the Cterminal tails of several transmembrane receptors, and ion channels. The C-terminal 57 amino acids region of syntrophins is highly conserved among the three isoforms, and may contain the binding site for dystrophin. Like members of the dystrophin family, syntrophins are expressed in a wide range of tissues. In rat skeletal muscle, alpha1 and beta1-syntrophins are localized on the sarcolemma, whereas beta2-syntrophin is largely restricted to the neuromuscular junction, associated with utrophin. Beta1-Syntrophin appears to be more restricted to fast twitch muscle fibers, the first fibers to degenerate in Duchenne muscular dystrophy. |
| Immunogen | Synthetic peptide corresponding to the N-terminal sequence of human alpha syntrophin (amino acids 2-18 with C-terminally added lysine) conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC: Use at a dilution of 1/100, this is determined by using paraformaldehyde-fixed, Triton X-100 treated, frozen sections of rat skeletal muscle. WB: Use at a dilution of 1/1000, this is determined using a membrane fraction extract of rat skeletal muscle homogenate. Staining of alpha 1 syntrophin is specifically inhibited with alpha 1 syntrophin immunizing peptide. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Syntrophin alpha1 Q13424 6640 SNTA1 Syntrophin alpha 1 601017 SNT1 dJ1187J4.5 TACIP1 |
|---|---|
| Target | Syntrophin alpha 1 |
| Cellular Localization | Membrane-associated. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions. |
| Application Notes | IHC: Use at a dilution of 1/100, this is determined by using paraformaldehyde-fixed, Triton X-100 treated, frozen sections of rat skeletal muscle. WB: Use at a dilution of 1/1000, this is determined using a membrane fraction extract of rat skeletal muscle homogenate. Staining of alpha 1 syntrophin is specifically inhibited with alpha 1 syntrophin immunizing peptide. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | Store at -20 degrees Celcius. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
