Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE alpha Tubulin antibody(Acetyl lys40) diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:200.| Product Name | alpha Tubulin antibody(Acetyl lys40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Tubulin(Acetyl lys40)Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary. |
| Modification | Acetyl lys40 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500~1:2000 *Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rat, Xenopus laevis, Sheep, Chimpanzee, BovineRat (100%), Xenopus laevis (92%), Sheep (100%), Chimpanzee (99%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ30169 antibody, H2-ALPHA antibody, TUBA1 antibody, TUBA4A antibody, tubulin H2-alpha antibody, |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cytoskeleton , Microtubule |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE alpha Tubulin antibody(Acetyl lys40) diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:200.| Positive Control | A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , mouse brain |
|---|---|
| Application Notes | WB:1:500~1:2000 *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
