Application
Human lung stained with in IHC paraffin sectionsApplication
This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin. This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Be| Product Name | alpha Tubulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin. |
| Immunogen | Synthetic peptide: MAALEK DYEEVGVDSV EGEGEEEGEE , corresponding to amino acids 426-450 of Human alpha Tubulin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| IHC-P: Use at a dilution of 1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMousePigRatGerbilGuinea PigNewtXenopusBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P68366, TUBA1, alpha Tubulin, 191110, H2ALPHA, H2-ALPHA, FLJ30169, 7277, H2 ALPHA |
|---|---|
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
Application
Human lung stained with in IHC paraffin sections
Application
This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin. This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Be| Positive Control | Lung tissue slides |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
