Application
Western blot of A431 cells, using Anti-α-Tubulin Clone RM113 at 1/1000 dilution, showed a band of α-Tubulin at the predicted MW (~50 kDa).Application
Immunocytochemical staining of HeLa cells, using Anti-α-Tubulin Clone RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).| Product Name | alpha tubulin antibody [RM113] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM113] to alpha tubulin |
| Immunogen | A peptide corresponding to the C-terminus of α-Tubulin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM113 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIPWB |
| Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Alpha tubulin |
|---|---|
| Tissue Specificity | This antibody reacts to α-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain. |
Application
Western blot of A431 cells, using Anti-α-Tubulin Clone RM113 at 1/1000 dilution, showed a band of α-Tubulin at the predicted MW (~50 kDa).
Application
Immunocytochemical staining of HeLa cells, using Anti-α-Tubulin Clone RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).| Application Notes | Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
