Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ALY antibody diluted at 1:500.Application
ALY antibody detects ALY protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ALY antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ALY antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
ALY antibody detects ALY protein at nucleus in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. ALY antibody diluted at 1:500.| Product Name | ALY antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALY (THO complex 4)The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALY. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Rhesus Monkey, BovineRat (82%), Chicken (83%), Rhesus Monkey (98%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALY antibody, ALY/REF antibody, BEF antibody, REF antibody, THOC4 antibody, ally of AML-1 and LEF-1 antibody, THO complex subunit 4 antibody, bZIP enhancing factor antibody, bZIP-enhancing factor BEF antibody, THO complex 4 antibody, tho4 antibody, transcr |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Nucleus , Nucleus speckle , Cytoplasm |
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ALY antibody diluted at 1:500.
Application
ALY antibody detects ALY protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ALY antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ALY antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
ALY antibody detects ALY protein at nucleus in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. ALY antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 , Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
