Application
IHC Image using - Detection of AMACR by IHC in Human Tonsil| Product Name | AMACR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AMACRAMARC has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMARC protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can be used as a positive marker for PIN. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 30 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | AMACR, 604489, 23600, RACE, Q9UHK6 |
|---|---|
| Target | AMACR |
| Cellular Localization | Mitochondrial and Peroxisomal. |
Application
IHC Image using - Detection of AMACR by IHC in Human Tonsil| Positive Control | Prostate carcinoma |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 30 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.2 containing 0.05% Sodium Azide |
