| Product Name | AMCR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AMCRAlpha-methyacyl-CoA racemase (AMCR), is an enzyme invloved in beta oxidation of branched chain fatty acids and bile salt intermediates, and has recently been identified as a neomarker for prostate cancer, where it is over expressed. Several different isoforms have been reported that are produced either by extensinve alternatve splicing of 5 exons or by use of alternate initiation codons. At least 2 different transcripts, each derived from the 5 exons, have been reported, AMCR I and AMCR II. AMCR I is the most abundant form and enclodes for a 382 amino acid protein (42kDa). The other isoform AMCR II exhibits significnat homolgy to fumerate hydratase and encoes a 288 amino acid protein (32 kDa). Several other variants of IA and IIA isoforms are charcterized recently. The variants lack exon 3 and are designated as IB and IIB. In prostate tumor tissues that over express AMCR, both the A and B forms are over-expressed. |
| Immunogen | Synthetic peptide: GAAVLRRLCKRSDVLLEPFR , corresponding to amino acids 62-81 of Human AMCR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IMWB |
| IM: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. GTX15893 strongly labels bands at 32 and 42 kDa corresponding to AMCR I and II variants. Does not work well with IHC. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMACR, AMCR, 604489, 23600, RACE, Q9UHK6 |
|---|---|
| Target | AMCR |
| Cellular Localization | Mitochondrial |
| Application Notes | IM: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. GTX15893 strongly labels bands at 32 and 42 kDa corresponding to AMCR I and II variants. Does not work well with IHC. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
