Application
Western blot analysis of extracts of various cell lines, using ACY1 antibody.Application
Immunofluorescence analysis of HeLa cell using ACY1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using ACY1 antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded rat kidney using ACY1 antibody at dilution of 1:100 (x40 lens).| Product Name | Aminoacylase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aminoacylase 1This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| Immunogen | Recombinant protein of human ACY1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:10 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACY1D , ACY1 , aminoacylase 1 |
|---|---|
| Target | Aminoacylase 1 |
Application
Western blot analysis of extracts of various cell lines, using ACY1 antibody.
Application
Immunofluorescence analysis of HeLa cell using ACY1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using ACY1 antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded rat kidney using ACY1 antibody at dilution of 1:100 (x40 lens).| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:10 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
