Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000Application
Aminoacylase 1 antibody [N2C3-2] detects Aminoacylase-1 protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. Aminoacylase 1 antibody [N2C3-2] dilution: 1:100.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Aminoacylase-1 antibody at 1:200 dilution.| Product Name | Aminoacylase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Aminoacylase 1Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Aminoacylase 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (85%), Rat (86%), Pig (86%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACY-1 antibody, ACY1D antibody, ACY1 antibody, acylase antibody, aminoacylase-1 antibody, N-acyl-L-amino-acid amidohydrolase antibody, aminoacylase 1 antibody |
|---|---|
| Target | Aminoacylase-1 |
| Molecular Weight(MW) | 46 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Application
Aminoacylase 1 antibody [N2C3-2] detects Aminoacylase-1 protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. Aminoacylase 1 antibody [N2C3-2] dilution: 1:100.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Aminoacylase-1 antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
