Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution: 1:500.Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 µg rat brain lysate/extract 5 % SDS-PAGE Amphiphysin antibody [N1N2], N-term dilution: 1:500Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 7.5 % SDS-PAGE Amphiphysin antibody [N1N2], N-term dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin antibody at 1:100 dilution.Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution: 1:500.Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution: 1:500.| Product Name | Amphiphysin antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Amphiphysin (amphiphysin)This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (84%), Pig (100%), Chicken (94%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPH1 antibody, AMPH antibody, amphiphysin antibody, amphiphysin I antibody, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) antibody |
|---|---|
| Molecular Weight(MW) | 76 kDa (note) (2) |
| Cellular Localization | Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Cytoplasm , cytoskeleton |
Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution: 1:500.
Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 µg rat brain lysate/extract 5 % SDS-PAGE Amphiphysin antibody [N1N2], N-term dilution: 1:500
Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 7.5 % SDS-PAGE Amphiphysin antibody [N1N2], N-term dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin antibody at 1:100 dilution.
Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution: 1:500.
Application
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution: 1:500.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
