| Product Name | Amphiregulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AmphiregulinAmphiregulin binds to EGF Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84 amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252 amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGF alpha, AR, HB EGF, cripto 1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas. |
| Immunogen | A synthetic 19 mer peptide, corresponding to amino acids 8 - 26 (VKPPQNKTESENTSDKPKR) from the secretedamphiregulin of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IP |
| Immunoprecipitation (with protein A, native): use at a concentration of 10μg / mg of lysate. Optimal dilutions / concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | MGC13647, Amphiregulin, CRDGF, AREG, 374, 104640, AR, P15514, SDGF |
|---|---|
| Target | Amphiregulin |
| Cellular Localization | Cytoplasmic and/or nuclear |
| Positive Control | LS174T cells |
|---|---|
| Application Notes | Immunoprecipitation (with protein A, native): use at a concentration of 10μg / mg of lysate. Optimal dilutions / concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
