Application
IMR32 whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Amphiregulin antibody detects Amphiregulin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Amphiregulin antibody diluted at 1:750.| Product Name | Amphiregulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Amphiregulin (amphiregulin)The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Amphiregulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AR antibody, AREGB antibody, CRDGF antibody, MGC13647 antibody, SDGF antibody, AREG antibody, schwannoma-derived growth factor antibody, colorectum cell-derived growth factor antibody, amphiregulin antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Membrane |
Application
IMR32 whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Amphiregulin antibody detects Amphiregulin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Amphiregulin antibody diluted at 1:750.| Positive Control | IMR32 , IMR32 membrane fraction extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
