Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody diluted at 1:500.Application
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) / AMPK alpha 2 (phospho Thr172) antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Product Name | AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) (protein kinase, AMP-activated, alpha 1 catalytic subunit)The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172). The exact sequence is proprietary. |
| Modification | p-Thr172 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (2) |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody diluted at 1:500.
Application
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) / AMPK alpha 2 (phospho Thr172) antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Positive Control | MCF-7(100μM CCCP treatment for 2 hr) , HeLa(500 μM CoCl2 treatment for 22 hr and 10 μM MG132 treatment for 2 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
