Application
Western blot analysis of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35ug/lane). PRKAA1 (arrow) was detected using the purified Pab.Application
PRKAA1 Antibody flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PRKAA1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRKAA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | AMPK alpha 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AMPK alpha 1The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | a recombinant protein from human PRKAA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 5'AMPactivated protein kinase catalytic subunit alpha1, AMPK subunit alpha1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, AMPK1, PRKAA1, PRKAA1 |
|---|---|
| Target | AMPK alpha 1 |
| Molecular Weight(MW) | 64.009 kDa (note) |
Application
Western blot analysis of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35ug/lane). PRKAA1 (arrow) was detected using the purified Pab.
Application
PRKAA1 Antibody flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PRKAA1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRKAA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.14% (W/V) sodium azide |
