Application
IP ImageAMPK alpha 1 antibody immunoprecipitates AMPK alpha 1 protein in IP experiments. IP samples: PC-3 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of AMPK alpha 1 protein by 4 μg AMPK alpha 1 antibody 7.5 % SDS-PAGE The immunoprecipitated AMPK alpha 1 protein was detected by AMPK alpha 1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG (-01) was used as a secondary reagent]Application
IHC-P ImageImmunohistochemical analysis of paraffin-embedded human gastric cancer, using AMPK alpha 1 antibody at 1:200 dilution.Application
WB ImageSample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Product Name | AMPK alpha 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK alpha 1 (protein kinase, AMP-activated, alpha 1 catalytic subunit)The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AMPK alpha 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineRat (100%), Zebrafish (97%), Xenopus laevis (97%), Pig (100%), Chicken (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, PRKAA1 antibody, AMPK alpha 1 antibody, tau-protein kinase PRKAA1 antibody, 5' -AMP-activated protein kinase catalytic subunit alpha-1 antibody, AMPK subunit alpha-1 antibody, |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (2) |
Application
IP ImageAMPK alpha 1 antibody immunoprecipitates AMPK alpha 1 protein in IP experiments. IP samples: PC-3 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of AMPK alpha 1 protein by 4 μg AMPK alpha 1 antibody 7.5 % SDS-PAGE The immunoprecipitated AMPK alpha 1 protein was detected by AMPK alpha 1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG (-01) was used as a secondary reagent]
Application
IHC-P ImageImmunohistochemical analysis of paraffin-embedded human gastric cancer, using AMPK alpha 1 antibody at 1:200 dilution.
Application
WB ImageSample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
