Application
Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKa1/AMPKa2(Ab-174/172) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPK alpha 1/AMPK alpha 2 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed Hela cells using AMPK alpha 1/AMPK alpha 2 Antibody .| Product Name | AMPK alpha 1/2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AMPK alpha 1/2Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. |
| Immunogen | Peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPK alpha 1/AMPK alpha 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMPK, AMPKa1, MGC33776, MGC57364, PRKAA1, AMPK alpha 1, tau-protein kinase PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, AMP-activated protein kinase, catalytic, alpha-1, 5'-AMP-activated protein kinase, catalyti |
|---|---|
| Target | AMPK alpha 1/2 |
| Molecular Weight(MW) | 63 (note) |
Application
Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKa1/AMPKa2(Ab-174/172) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPK alpha 1/AMPK alpha 2 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed Hela cells using AMPK alpha 1/AMPK alpha 2 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
