Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody diluted at 1:1000.Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AMPK alpha 2 protein stained by AMPK alpha 2 antibody diluted at 1:400. Blue: Hoechst 33342 staining.Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. AMPK alpha 2 antibody diluted at 1:500.Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. AMPK alpha 2 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 μg of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | AMPK alpha 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Sheep, BovineRat (98%), Pig (99%), Chicken (96%), Sheep (98%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPK antibody, AMPK2 antibody, PRKAA antibody, PRKAA2 antibody, AMPK-alpha-2 chain antibody, 5'-AMP-activated protein kinase, catalytic alpha-2 chain antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody, AMPK subunit alpha-2 antibod |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody diluted at 1:1000.
Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AMPK alpha 2 protein stained by AMPK alpha 2 antibody diluted at 1:400. Blue: Hoechst 33342 staining.
Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. AMPK alpha 2 antibody diluted at 1:500.
Application
AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. AMPK alpha 2 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 μg of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Positive Control | A431 , HeLa , A375 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
