Application
Application| Product Name | AMPK alpha 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AMPK alpha 2The AMP-activated protein kinase (AMPK) in mammals, and its homologue in Saccharomyces cerevisiae, are activated by cellular stresses associated with ATP depletion. AMPK is a heterotrimer comprising a catalytic alpha subunit with associated beta and gamma subunits.The alpha subunit has at least two isoforms (alpha 1 and alpha 2), which differ in their AMP-dependence and subcellular localization, with alpha 2 complexes being partly nuclear. AMPK is activated allosterically by 5'-AMP, which also promotes phosphorylation and activation by an upstream kinase, and inhibits dephosphorylation and inactivation.Elevation of AMP always accompanies depletion of ATP due to the action of adenylate kinase. Since high ATP antagonizes the activating effects of AMP, the system behaves like a cellular 'fuel gauge'. It is activated by various types of stress associated with ATP depletion, such as hypoxia, heat shock, metabolic poisoning and, in muscle, exercise. AMPK phosphorylates multiple targets which switch off anabolic pathways and switch on alternative catabolic pathways. |
| Immunogen | Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at a concentration of 2 - 8 μg/ml. For WB: Use at a dilution of 0.2 - 2μg/ml. Predicted molecular weight: 63 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 5563, AMPK alpha 2, PRKAA2, AMPK, AMPK alpha2, 600497, PRKAA, AMPK2, P54646 |
|---|---|
| Target | AMPK alpha 2 |
Application
Application| Application Notes | For IP: Use at a concentration of 2 - 8 μg/ml. For WB: Use at a dilution of 0.2 - 2μg/ml. Predicted molecular weight: 63 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.1ml Tris-citrate/phosphate, pH 7-8, with 0.1% sodium azide |
