Application
WB analysis of extracts from Jurkat cells using AMPK beta1 (phospho Ser182) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
IHC-P analysis of human lung carcinoma tissue using AMPK beta1 (phospho Ser182) antibody in the absence (left) or presence (right) of blocking peptide.| Product Name | AMPK beta 1 (phospho Ser182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AMPK beta 1 (phospho Ser182)The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq] |
| Modification | p-Ser182 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMPK, HAMPKb, MGC17785 |
|---|---|
| Target | AMPK beta 1 (phospho Ser182) |
Application
WB analysis of extracts from Jurkat cells using AMPK beta1 (phospho Ser182) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
IHC-P analysis of human lung carcinoma tissue using AMPK beta1 (phospho Ser182) antibody in the absence (left) or presence (right) of blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
