Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using AMPK beta 1 antibody at 1:300 dilution.| Product Name | AMPK beta 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK beta 1 (protein kinase, AMP-activated, beta 1 non-catalytic subunit)The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AMPK beta 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (97%), Rat (97%), Xenopus laevis (89%), Pig (97%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMPK antibody, HAMPKb antibody, MGC17785 antibody, PRKAB1 antibody, AMPKb antibody, protein kinase, AMP-activated, noncatalytic, beta-1 antibody, AMPK beta -1 chain antibody, 5'-AMP-activated protein kinase beta-1 subunit antibody, AMPK beta 1 antibody, 5' |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) |
Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using AMPK beta 1 antibody at 1:300 dilution.| Positive Control | HepG2 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
