Application
Western blot analysis of extracts of various cell lines, using PRKAB2 antibody.Application
Immunofluorescence analysis of U20S cell using PRKAB2 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded rat kidney using PRKAB2 antibody at dilution of 1:100 (x40 lens).| Product Name | AMPK beta 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK beta 2The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human PRKAB2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | protein kinase, AMP-activated, beta 2 non-catalytic subunit , PRKAB2 |
|---|---|
| Target | AMPK beta 2 |
| Molecular Weight(MW) | 30 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using PRKAB2 antibody.
Application
Immunofluorescence analysis of U20S cell using PRKAB2 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded rat kidney using PRKAB2 antibody at dilution of 1:100 (x40 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
