Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AMPK gamma-2 antibody at 1:200 dilution.Application
AMPK gamma 2 antibody detects AMPK gamma 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AMPK gamma 2 antibody at a dilution of 1:1000.Application
AMPK gamma 2 antibody detects PRKAG2 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer tissue. AMPK gamma 2 antibody dilution: 1:500.| Product Name | AMPK gamma 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK gamma 2 (protein kinase, AMP-activated, gamma 2 non-catalytic subunit)AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Full length human AMPK gamma 2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChickenMouse (98%), Rat (97%), Chicken (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AAKG antibody, AAKG2 antibody, CMH6 antibody, H91620p antibody, WPWS antibody, PRKAG2 antibody, AMPK gamma2 antibody, AMPK subunit gamma-2 antibody, 5'-AMP-activated protein kinase subunit gamma-2 antibody, protein kinase, AMP-activated, gamma 2 non-cataly |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) (5) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AMPK gamma-2 antibody at 1:200 dilution.
Application
AMPK gamma 2 antibody detects AMPK gamma 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AMPK gamma 2 antibody at a dilution of 1:1000.
Application
AMPK gamma 2 antibody detects PRKAG2 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer tissue. AMPK gamma 2 antibody dilution: 1:500.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
