Application
WB analysis of extract from HeLa cells untreated or treated with heat shock (30min) using AMPK1/2 (phospho Ser485/Ser491) antibody.Application
IHC-P analysis of human colon carcinoma tissue using AMPK1/2 (phospho Ser485/Ser491) antibody in the absence (left) or presence (right) of blocking peptide.| Product Name | AMPK1/AMPK2 (phospho Ser485/Ser491) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AMPK1/AMPK2 (phospho Ser485/Ser491)The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq] |
| Modification | p-Ser485/Ser491 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMPK, AMPK2, PRKAA |
|---|---|
| Target | AMPK1/AMPK2 (phospho Ser485/Ser491) |
Application
WB analysis of extract from HeLa cells untreated or treated with heat shock (30min) using AMPK1/2 (phospho Ser485/Ser491) antibody.
Application
IHC-P analysis of human colon carcinoma tissue using AMPK1/2 (phospho Ser485/Ser491) antibody in the absence (left) or presence (right) of blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
