Application
Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.Application
AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | AMT antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AMT (N-term)The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.1), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; GLDC, MIM 2383), H protein (a lipoic acid-containing protein; GCSH, MIM 23833), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; DLD, MIM 238331). Mutations in the T, P, and H proteins have been found to cause glycine encephalopathy (GCE; MIM 65899).[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide between 25~54 amino acids from the N-terminal region of human AMT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | GCVT, GCST, Aminomethyltransferase, mitochondrial, AMT, Glycine cleavage system T protein |
|---|---|
| Target | AMT |
| Molecular Weight(MW) | 43.946 kDa (note) |
Application
Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.
Application
AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
