Application
Human Pancreatic Carcinoma (formalin-fixed, paraffin-embedded) stained with ANAPC1 antibody at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.Application
Western blot of affinity purified anti-APC1 pS377 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature.| Product Name | ANAPC1 (phospho Ser377) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ANAPC1 (phospho Ser377)ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.[supplied by OMIM] |
| Immunogen | Synthetic peptide |
| Modification | p-Ser377 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB (1:200 - 1:1000), ELISA (1:2000 - 1:10000), IF, IHC-P (10 µg/ml) | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANAPC1 (phospho S377), ANAPC1 (phospho Ser377), ANAPC1 (pS377), ANAPC1 (pSer377), ANAPC1 phospho S377, ANAPC1 phospho Ser377, Phospho ANAPC1 (S377), Phospho ANAPC1 (Ser377), Phospho ANAPC1 (pS377), Phospho ANAPC1 (pSer377) |
|---|---|
| Target | ANAPC1 |
| Tissue Specificity | Amino acids 373-382 of Human Apc1 protein. |
Application
Human Pancreatic Carcinoma (formalin-fixed, paraffin-embedded) stained with ANAPC1 antibody at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Application
Western blot of affinity purified anti-APC1 pS377 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature.| Application Notes | WB (1:200 - 1:1000), ELISA (1:2000 - 1:10000), IF, IHC-P (10 µg/ml) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
