Application
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1, DU145 sections.Application
Western Blot analysis of 22RV1, DU145, and PC-3 whole cell lysates, using anti-AR-V7 rabbit monoclonal Antibody Clone RM7, showed endogenous AR-V7 expressed in 22RV1 cells.Application
Immunocytochemical staining of 22RV1 and PC-3 cells,using anti-AR-V7 rabbit monoclonal Antibody Clone RM7 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Androgen Receptor (ARv7 Splice Variant) antibody [RM7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM7] to Androgen Receptor (ARv7 Splice Variant)The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to AR splice variant 7 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM7 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Immunohistochemistry (IHC): 1:100 – 1:200 dilution, Immunocytochemistry (ICC): 1:100 – 1:1000 dilution, Western Blot (WB): 1:500 – 1:1000 dilution. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SMAX1 , AR , HYSP1 , TFM , DHTR , HUMARA , KD , AIS , androgen receptor , NR3C4 , SBMA |
|---|---|
| Target | Androgen Receptor (ARv7 Splice Variant) |
| Tissue Specificity | This antibody reacts to androgen receptor splice variant 7 (AR-V7). No cross reactivity with wild type androgen receptor. |
Application
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1, DU145 sections.
Application
Western Blot analysis of 22RV1, DU145, and PC-3 whole cell lysates, using anti-AR-V7 rabbit monoclonal Antibody Clone RM7, showed endogenous AR-V7 expressed in 22RV1 cells.
Application
Immunocytochemical staining of 22RV1 and PC-3 cells,using anti-AR-V7 rabbit monoclonal Antibody Clone RM7 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | Immunohistochemistry (IHC): 1:100 – 1:200 dilution, Immunocytochemistry (ICC): 1:100 – 1:1000 dilution, Western Blot (WB): 1:500 – 1:1000 dilution. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
