Application
Immunofluorescence staining of methanol-fixed Hela cells using Androgen Receptor(Phospho-Ser213)Application
Western blot analysis of extracts from DU145 cells using Androgen Receptor(Phospho-Ser213) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Product Name | Androgen Receptor (phospho Ser213) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Androgen Receptor (Phospho Ser213)The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. |
| Immunogen | Peptide sequence around phosphorylation site of serine 213 (E-A-S(p)-G-A) derived from Human Androgen Receptor. |
| Modification | p-Ser213 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, AR, dihydrotestosterone receptor, androgen receptor, androgen nuclear receptor variant 2, nuclear receptor subfamily 3 group C member 4 |
|---|---|
| Target | Androgen Receptor (Phospho Ser213) |
| Molecular Weight(MW) | 110 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Androgen Receptor(Phospho-Ser213)
Application
Western blot analysis of extracts from DU145 cells using Androgen Receptor(Phospho-Ser213) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
