Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution.Application
Angiopoietin 2 antibody detects Angiopoietin 2 protein by western blot analysis. Mouse lung extracts (50 µg) and HUVEC cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Angiopoietin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Angiopoietin 2 (angiopoietin-2)The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Angiopoietin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, BovineRat (86%), Dog (92%), Pig (92%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AGPT2 antibody ANG2 antibody ANGPT2 antibody ANG-2 antibody Tie2-ligand antibody angiopoietin-2B antibody angiopoietin-2a antibody angiopoietin-2 antibody angiopoietin 2 antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution.
Application
Angiopoietin 2 antibody detects Angiopoietin 2 protein by western blot analysis. Mouse lung extracts (50 µg) and HUVEC cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | mouse lung , HUVEC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
