Application
IHC-P of anti-ANG2 Antibody in human placenta tissue. Antigen Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml for 1.5 hour RT.Application
Western blotting. Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells. Lane 1 : mock endothelial cells. Lane 2 : mouse Ang-2 expressing cells. Lane 3 - mouse Ang-1 expressing cells. Lane 4 - mouse Ang-1 expressing cells. Lane 5 - wt endothelial cells. Approximately 20 µg of each lysate was loaded for 10% SDS-PAGE.| Product Name | Angiopoietin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Angiopoietin 2Angiopoietin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-1 antibody, GT28451) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD. |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AGPT2 , ANGPT2 , ANG2 |
|---|---|
| Target | Angiopoietin 2 |
| Tissue Specificity | This antibody is specific for mouse Angiopoietin 2 (mAng-2). Partially cross-reactivity is noted with human angiopoietin-1 |
Application
IHC-P of anti-ANG2 Antibody in human placenta tissue. Antigen Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml for 1.5 hour RT.
Application
Western blotting. Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells. Lane 1 : mock endothelial cells. Lane 2 : mouse Ang-2 expressing cells. Lane 3 - mouse Ang-1 expressing cells. Lane 4 - mouse Ang-1 expressing cells. Lane 5 - wt endothelial cells. Approximately 20 µg of each lysate was loaded for 10% SDS-PAGE.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide |
