Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANGPTL3 antibody at 1:500 dilution.Application
HepG2 conditioned medium (10 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ANGPTL3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ANGPTL3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ANGPTL3 (angiopoietin-like 3)The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ANGPTL3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Pig, BovineMouse (80%), Pig (85%), Bovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANGPT5 antibody, FHBL2 antibody, ANGPTL3 antibody, ANG-5 antibody, angiopoietin-related protein 3 antibody, angiopoietin 5 antibody, angiopoietin-like 3 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANGPTL3 antibody at 1:500 dilution.
Application
HepG2 conditioned medium (10 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ANGPTL3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 conditioned medium |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
