Application
Immunohistochemical analysis of paraffin-embedded human Kidney, using Anillin antibody at 1:100 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Anillin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term diluted at 1:500.| Product Name | Anillin antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Anillin (anillin, actin binding protein)Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Anillin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779A055 antibody, Scraps antibody, scra antibody, ANLN antibody, actin-binding protein anillin antibody, anillin, actin binding protein antibody |
|---|---|
| Molecular Weight(MW) | 124 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm , cytoskeleton , cell cortex |
Application
Immunohistochemical analysis of paraffin-embedded human Kidney, using Anillin antibody at 1:100 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Anillin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term diluted at 1:500.| Positive Control | 293T , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
