Application
Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.Application
Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II antibody at 1:200 dilution.Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg HCT116 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody antibody at 1:500 dilution.| Product Name | Annexin II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin II (annexin A2)This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin II. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Chicken, Sheep, BovineXenopus laevis (81%), Dog (100%), Pig (100%), Chicken (90%), Sheep (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANX2 antibody ANX2L4 antibody CAL1H antibody LIP2 antibody LPC2 antibody LPC2D antibody P36 antibody PAP-IV antibody ANXA2 antibody placental anticoagulant protein IV antibody calpactin I heavy polypeptide antibody annexin II antibody lipocorti |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (4) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome |
Application
Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.
Application
Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II antibody at 1:200 dilution.
Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000
Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000
Application
Annexin II antibody detects ANXA2 protein by Western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg HCT116 whole cell lysate/extract 10 % SDS-PAGE Annexin II antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody antibody at 1:500 dilution.| Positive Control | A549 , HCT116 , HeLa , GL261 , NIH-3T3 , C2C12 , Rat2 , human ANXA2-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
