Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Annexin IV antibody diluted at 1:1000Application
Immunoprecipitation of Annexin IV protein from HepG2 whole cell extracts using 5 μg of Annexin IV antibody . Western blot analysis was performed using Annexin IV antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Annexin IV antibody detects ANXA4 protein at cytosol and membrane on D54MG xenograft by immunohistochemical analysis. Sample: Paraffin-embedded D54MG xenograft. Annexin IV antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Annexin A4 antibody at 1:200 dilution.| Product Name | Annexin IV antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin IV (annexin A4)Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin IV. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rhesus Monkey, BovineMouse (91%), Rat (92%), Dog (93%), Pig (96%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANX4 antibody, DKFZp686H02120 antibody, MGC75105 antibody, PIG28 antibody, ZAP36 antibody, ANXA4 antibody, carbohydrate-binding protein p33/p41 antibody, endonexin I antibody, annexin A4 antibody, 35-beta calcimedin antibody, PP4-X antibody, annexin IV (pl |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Annexin IV antibody diluted at 1:1000
Application
Immunoprecipitation of Annexin IV protein from HepG2 whole cell extracts using 5 μg of Annexin IV antibody . Western blot analysis was performed using Annexin IV antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Annexin IV antibody detects ANXA4 protein at cytosol and membrane on D54MG xenograft by immunohistochemical analysis. Sample: Paraffin-embedded D54MG xenograft. Annexin IV antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Annexin A4 antibody at 1:200 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
