Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw 264.7 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin V antibody at 1:500 dilution.Application
Annexin V antibody detects Annexin V protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Annexin V antibody diluted at 1:500.| Product Name | Annexin V antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin V (annexin A5)The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Chimpanzee, BovineDog (98%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANX5 antibody, ENX2 antibody, PP4 antibody, ANXA5 antibody, thromboplastin inhibitor antibody, annexin V antibody, lipocortin V antibody, VAC-alpha antibody, anchorin CII antibody, endonexin II antibody, annexin-5 antibody, placental anticoagulant protein |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw 264.7 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin V antibody at 1:500 dilution.
Application
Annexin V antibody detects Annexin V protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Annexin V antibody diluted at 1:500.| Positive Control | A431 , HeLa , HepG2 , GL261 , C8D30 , NIH-3T3 , Raw 264.7 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
