Application
Annexin VII antibody detects ANXA7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin VII antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Annexin VII antibody detects Annexin VII protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 10 % SDS-PAGE Annexin VII antibody dilution: 1:1000Application
Annexin VII antibody detects Annexin VII protein at cytosol on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. Annexin VII antibody dilution: 1:500.| Product Name | Annexin VII antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin VII (annexin A7)Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin VII. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (94%), Rat (94%), Chicken (85%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANX7 antibody SNX antibody SYNEXIN antibody ANXA7 antibody annexin A7 antibody annexin-7 antibody annexin VII antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (4) |
Application
Annexin VII antibody detects ANXA7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin VII antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Annexin VII antibody detects Annexin VII protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 10 % SDS-PAGE Annexin VII antibody dilution: 1:1000
Application
Annexin VII antibody detects Annexin VII protein at cytosol on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. Annexin VII antibody dilution: 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
