Application
ANP antibody detects ANP protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ANP antibody [N1C3] detects ANP protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ANP antibody [N1C3] diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANP antibody at 1:500 dilution.| Product Name | ANP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ANP (natriuretic peptide precursor A)The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq] |
| Immunogen | Full length human ANP Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Rabbit, BovineDog (83%), Pig (87%), Rabbit (81%), Bovine (83%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANF antibody, ANP antibody, ATFB6 antibody, CDD-ANF antibody, PND antibody, NPPA antibody, cardionatrin antibody, natriuretic peptide precursor A antibody, prepronatriodilatin antibody, natriuretic peptides A antibody, atriopeptin antibody, natriuretic pep |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) |
| Cellular Localization | Secreted |
Application
ANP antibody detects ANP protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ANP antibody [N1C3] detects ANP protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ANP antibody [N1C3] diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANP antibody at 1:500 dilution.| Positive Control | mouse heart , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
